METHOD FOR PREPARING VIRUS-LIKE PARTICLES USING A PROKARYOTIC EXPRESSION SYSTEM

A method for preparing virus-like particles through a prokaryotic expression system comprises: l) inoculation;2) bacteria culture: 2.1) adding a carbon source culture medium to a batch culture medium to maintain the pH of the bacteria suspension within a range of 6.95- 7.05; where the carbon source culture medium comprises a carbon source and an ammonium salt of strong acid; and2.2) adding a nitrogen source culture medium to the batch culture medium when the bacteria suspension reaches an OD600 of 7-10; 3) low-temperature culture; 4) induction expression of protein; and 5) purification and assembly. The method employs E. coli prokaryotic expression system as a carrier, and performs fed-batch culture by adding the nitrogen source and the carbon soruce in a C/N ratio at a feed rate, thus increasing the growth of the E. coli.

A method for preparing virus-like particles through a prokaryotic expression system comprises: l) inoculation;2) bacteria culture: 2.1) adding a carbon source culture medium to a batch culture medium to maintain the pH of the bacteria suspension within a range of 6.95- 7.05; where the carbon source culture medium comprises a carbon source and an ammonium salt of strong acid; and2.2) adding a nitrogen source culture medium to the batch culture medium when the bacteria suspension reaches an OD600 of 7-10; 3) low-temperature culture; 4) induction expression of protein; and 5) purification and assembly. The method employs E. coli prokaryotic expression system as a carrier, and performs fed-batch culture by adding the nitrogen source and the carbon soruce in a C/N ratio at a feed rate, thus increasing the growth of the E. coli.